On the value of Brücke's method for the removal of interfering substances from urine in testing for glucose / by Edgar Moore Green.
- Edgar Moore Green
- Date:
- [1886]
Licence: Public Domain Mark
Credit: On the value of Brücke's method for the removal of interfering substances from urine in testing for glucose / by Edgar Moore Green. Source: Wellcome Collection.
Provider: This material has been provided by The Royal College of Surgeons of England. The original may be consulted at The Royal College of Surgeons of England.
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![[Reprinted from American Chemical Journal, Vol. VIII, No. 2.] ON THE VALUE OF BRUCKE'S METHOD FOR THE REMOVAL OF INTERFERING SUBSTANCES FROM URINE IN TESTING FOR GLUCOSE. By Edgar Moore Green. In determining the presence of glucose in diabetic urine by any of the copper tests it is found that, under certain circumstances, some of the normal constituents of urine, such as creatinine and uric acid, interfere with the reaction of these tests. In the ordinary course of a case of diabetes, when the urine contains an abundance of glucose, usually no difficulty is experienced in obtaining the reactions; but after the patient has been restricted to a diet of nitrogenous food alone, great difficulty is often found in making the tests, because of a diminution in the amount of glucose and an increase in the amount of the interfering substances, creatinine, uric acid, etc. It may therefore be necessary to remove these substances before testing for the glucose. The method for their removal suggested by Briicke, and in use many years, con- sisted in adding neutral plumbic acetate, Pb(GH302)2, to the urine, and immediately afterward adding plumbic tribasic acetate, Pb*02(C2H30'2)2. The precipitate was then separated by filtra- tion and washed with distilled water. The filtrate and washings having been collected in one vessel, ammonium hydroxide was added, which threw down the glucose in combination with the lead in the form of lead saccharate, (PbO^CeH^OsX This white precipitate was collected on a filter paper, washed, and then suspended in distilled water, and hydrogen sulphide, which decomposed the compound, passed through it. The filtered solution contained the glucose ;* and it was used for the tests, after the hydrogen sulphide had been driven off by evaporation.'2 The original paper by Briicke could not be obtained, as it was not to be found in the journal referred to by Neubauer and Vogel. How- ever, Beale gives the same method as the preceding. According 1 The neutral plumbic acetate removes the sulphates, phosphates, and carbonates. The plumbic tribasic acetate precipitates the coloring matter, and at least a portion of the uric acid and creatinine. Probably a small amount of uric acid is contained in the precipitate of lead saccharate, and the remainder of the uric acid and creatinine, together with the urea, are con- tained in the filtrate after removal of the lead saccharate.](https://iiif.wellcomecollection.org/image/b22275423_0003.jp2/full/800%2C/0/default.jpg)
